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KMID : 1161420170200121184
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Journal of Medicinal Food
2017 Volume.20 No. 12 p.1184 ~ p.1192
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The Edible Mushroom Pleurotus djamor Produces Metabolites with Lethal Activity Against the Parasitic Nematode Haemonchus contortus
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Pineda-Alegria Jesus Antonio
Sanchez-Vazquez Jose Ernesto
Gonzalez-Cortazar Manases
Zamilpa Alejandro
Lopez-Arellano Maria Eugenia
Cuevas-Padilla Edgar Josue
Mendoza-de-Gives Pedro
Aguilar-Marcelino Liliana
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Abstract
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Pleurotus djamor is an edible mushroom that has medicinal properties. This study aimed to assess the in vitro activity of P. djamor extracts and fractions against Haemonchus contortus eggs and exsheathed infective larvae (L3). Crude hydroalcoholic extracts were obtained by maceration and fractions were obtained through chromatography. Metabolite identity was determined using gas chromatography/mass spectrometry (GC-MS) analysis. The results showed that P. djamor extracts had no significant activity against eggs at the concentrations used. However, the extract showed 98.7% and 77% larval mortality 72?h postconfrontation at 320 and at 160?mg/mL, respectively. The chromatography analysis resulted in 23 fractions that were eventually grouped into three fractions (E1, E2, and E3). These fractions showed the following egg hatching inhibition percentages: E1?=?100, E2?=?38.7, and E3?=?5.5 at 10?mg/mL concentration 72?h postexposure. Likewise, larval mortality percentages after this period were 90.6, 100, and 0.44 at 40?mg/mL (P?
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KEYWORD
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fatty acids, GC-MS chromatography, Haemonchus contortus, ovine nematodesPleurotus djamor, terpene
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